Hotspot

trnC-GCA-petN

LSC · 28425-29132 · 708 bp

Back to Pseudogalium

Pi 0.00282

MarkerSeek score 50.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 3
pi: 0.00282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	739-764 bp	1.0	81.1
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TCCACTTCTTCCCCACACTA	50.0	50.0	58.1	57.6	796-821 bp	1.0	78.8
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	757-782 bp	1.0	78.8
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAC	50.0	55.0	58.1	58.6	800-825 bp	1.0	76.9
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TCCACTTCTTCCCCACACTA	50.0	50.0	58.4	57.6	814-839 bp	1.0	76.6