Hotspot

rps16-trnQ-UUG

LSC · 6049-7455 · 1407 bp

Back to Pseudogalium

Pi 0.0118

MarkerSeek score 57.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1407
variable_sites: 25
pi: 0.0118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1470-1507 bp	1.0	80.9
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1474-1511 bp	1.0	76.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1500-1537 bp	1.0	76.0
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1462-1499 bp	1.0	75.7
rps16-trnQ-UUG_p5	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.9	58.1	1469-1506 bp	1.0	75.1