Hotspot

ndhF

SSC · 113151-115322 · 2172 bp

Back to Pseudodissochaeta

Pi 0.00645

MarkerSeek score 47.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2172
variable_sites: 14
pi: 0.00645
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTTGAATGGTCCCTATCGCG	TCGATTGCACTATTAGCCTGA	50.0	42.9	58.1	56.9	2605 bp	1.0	63.3
ndhF_p2	CCTATCGCGGAAGGAGAAAC	TCGATTGCACTATTAGCCTGA	55.0	42.9	58.1	56.9	2594 bp	1.0	62.5
ndhF_p3	TCGCGGAAGGAGAAACATTT	TCGATTGCACTATTAGCCTGA	45.0	42.9	57.8	56.9	2590 bp	1.0	62.3
ndhF_p4	TTTGAATGGTCCCTATCGCG	TGCACTATTAGCCTGATCCT	50.0	45.0	58.1	55.7	2600 bp	1.0	61.6
ndhF_p5	CCTATCGCGGAAGGAGAAAC	TGCACTATTAGCCTGATCCT	55.0	45.0	58.1	55.7	2589 bp	1.0	60.9