Hotspot

psbE-petL

LSC · 66214-67355 · 1142 bp

Back to Pseudodissochaeta

Pi 0.00616

MarkerSeek score 62.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1142
variable_sites: 7
pi: 0.00616
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	TGGGTTGTGCTCATTCAATTG	50.0	42.9	57.8	57.3	1284-1289 bp	1.0	66.2
psbE-petL_p2	ACGAATAACCAACCCGCAAT	TGGGTTGTGCTCATTCAATTG	45.0	42.9	58.2	57.3	1370-1375 bp	1.0	66.0
psbE-petL_p3	TGCTGACGAATAACCAACCC	TGGGTTGTGCTCATTCAATTG	50.0	42.9	58.2	57.3	1375-1380 bp	1.0	65.9
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	TGGGTTGTGCTCATTCAATTG	55.0	42.9	58.2	57.3	1279-1284 bp	1.0	65.9
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	TGGGTTGTGCTCATTCAATTG	50.0	42.9	58.4	57.3	1277-1282 bp	1.0	64.6