Hotspot

psaI-ycf4

LSC · 61269-61707 · 439 bp

Back to Pseudodissochaeta

Pi 0.019

MarkerSeek score 64.80

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 439
variable_sites: 8
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	TGTTCGGATCGCAAACTCAT	45.0	45.0	58.0	57.9	504-520 bp	1.0	82.9
psaI-ycf4_p2	GGCAATTGCAATGGCTTCTT	TGTTCGGATCGCAAACTCAT	45.0	45.0	57.9	57.9	498-514 bp	1.0	82.4
psaI-ycf4_p3	ATTTCCGGCAATTGCAATGG	TCTGTTCGGATCGCAAACTC	45.0	50.0	58.0	58.3	506-522 bp	1.0	81.4
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	AGGATCAAGGCCCAGAAGAA	45.0	50.0	58.0	58.3	567-583 bp	1.0	80.7
psaI-ycf4_p5	ATTTCCGGCAATTGCAATGG	AAGGATCAAGGCCCAGAAGA	45.0	50.0	58.0	58.3	568-584 bp	1.0	80.6