Hotspot

ndhC-trnV-UAC

LSC · 52432-52933 · 502 bp

Back to Pseudodissochaeta

Pi 0.014

MarkerSeek score 64.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 502
variable_sites: 7
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGACCTTTACTAATCGGGGC	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.7	57.9	636-637 bp	1.0	78.4
ndhC-trnV-UAC_p2	AATCGGGGCTAAAATTCCGG	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	625-626 bp	1.0	77.9
ndhC-trnV-UAC_p3	GGACCTTTACTAATCGGGGC	CGGTTCGAATCCGTATAGCC	55.0	55.0	57.7	58.3	637-638 bp	1.0	76.5
ndhC-trnV-UAC_p4	CGGGGCTAAAATTCCGGAAA	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.5	57.9	622-623 bp	1.0	76.1
ndhC-trnV-UAC_p5	AATCGGGGCTAAAATTCCGG	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.3	58.3	626-627 bp	1.0	76.1