Hotspot

psaA-ycf3

LSC · 43522-44117 · 596 bp

Back to Pseudodissochaeta

Pi 0.00671

MarkerSeek score 62.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 596
variable_sites: 4
pi: 0.00671
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	774 bp	1.0	79.2
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	756 bp	1.0	79.1
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AAGATCACAAGGCGTTTCGA	50.0	45.0	57.9	57.8	700 bp	1.0	78.2
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGATCACAAGGCGTTTCGAA	50.0	45.0	57.9	57.8	699 bp	1.0	78.2
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	770 bp	1.0	77.4