Hotspot

atpF-atpH

LSC · 13342-13959 · 618 bp

Back to Pseudodissochaeta

Pi 0.0131

MarkerSeek score 54.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 618
variable_sites: 8
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AAAGGAAAGAATCGGTTAGAT	CTGTTATTTGCGAATCCCTTTGT	33.3	39.1	52.5	57.9	669-673 bp	1.0	42.0
atpF-atpH_p2	AAAGGAAAGAATCGGTTAGAT	TGTTATTTGCGAATCCCTTTGT	33.3	36.4	52.5	56.9	668-672 bp	1.0	42.0
atpF-atpH_p3	AAAGGAAAGAATCGGTTAGAT	TGTTATTTGCGAATCCCTTTGTT	33.3	34.8	52.5	57.5	668-672 bp	1.0	42.0
atpF-atpH_p4	AAAGGAAAGAATCGGTTAGAT	ATTTGCGAATCCCTTTGTTT	33.3	35.0	52.5	54.3	664-668 bp	1.0	42.0
atpF-atpH_p5	AAAGGAAAGAATCGGTTAGAT	CTGTTATTTGCGAATCCCTTTG	33.3	40.9	52.5	56.3	669-673 bp	1.0	42.0