Hotspot

psbI-trnS-GCU

LSC · 7956-8021 · 66 bp

Back to Pseudodissochaeta

Pi 0.0303

MarkerSeek score 63.50

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 2
pi: 0.0303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATCCCGGACGTGACGAATA	TTTTTCGTACCGAGGGTTCG	50.0	50.0	58.9	58.2	123 bp	1.0	72.4
psbI-trnS-GCU_p2	ATCCCGGACGTGACGAATAA	TTTTTCGTACCGAGGGTTCG	50.0	50.0	58.9	58.2	122 bp	1.0	72.4
psbI-trnS-GCU_p3	TAATCCCGGACGTGACGAAT	TTTTTCGTACCGAGGGTTCG	50.0	50.0	58.9	58.2	124 bp	1.0	72.4
psbI-trnS-GCU_p4	AATCCCGGACGTGACGAATA	CCTAAAGCGTCGGATTGCTA	50.0	50.0	58.9	57.8	157 bp	1.0	72.1
psbI-trnS-GCU_p5	ATCCCGGACGTGACGAATAA	CCTAAAGCGTCGGATTGCTA	50.0	50.0	58.9	57.8	156 bp	1.0	72.1