Hotspot

psbA

LSC · 437-1516 · 1080 bp

Back to Pseudocodon

Pi NA

MarkerSeek score 46.20

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1080
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCACAATCCACTGCCTTGTC	TTCGGTAGAGTCAAGTGGGT	50.0	50.0	58.1	58.0	1668 bp	0.5	59.1
psbA_p2	TCCGCCCCTCTACTATGTTT	TTCGGTAGAGTCAAGTGGGT	50.0	50.0	57.8	58.0	1636 bp	0.5	58.6
psbA_p3	TCACAATCCACTGCCTTGTC	CCGCGAAAGTCTGCTAGAAT	50.0	50.0	58.1	58.1	1648 bp	0.5	58.6
psbA_p4	TCACAATCCACTGCCTTGTC	AAGCACGAAAGCGAAGATCT	50.0	45.0	58.1	57.9	1943 bp	0.5	58.5
psbA_p5	TCACAATCCACTGCCTTGTC	AGCACGAAAGCGAAGATCTT	50.0	45.0	58.1	57.9	1942 bp	0.5	58.5