Hotspot

ndhF-trnL-UAG

SSC · 137227-141497 · 4271 bp

Back to Pseudocodon

Pi 0.00213

MarkerSeek score 49.10

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 4271
variable_sites: 5
pi: 0.00213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.55
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	AGGTTCAGTCTCCGATTCCT	TTTCGTTCTATACCGGCACC	50.0	50.0	57.8	57.7	223 bp	1.0	76.8
ndhF-trnL-UAG_p2	ACTCAAATGATCCACGGCTG	TTTCGTTCTATACCGGCACC	50.0	50.0	58.3	57.7	290 bp	1.0	76.4
ndhF-trnL-UAG_p3	CACTCAAATGATCCACGGCT	TTTCGTTCTATACCGGCACC	50.0	50.0	58.3	57.7	291 bp	1.0	76.4
ndhF-trnL-UAG_p4	ACGGCTGCCAATATATCTCG	TTTCGTTCTATACCGGCACC	50.0	50.0	57.6	57.7	277 bp	1.0	75.5
ndhF-trnL-UAG_p5	CTGCCAATATATCTCGCGGT	TTTCGTTCTATACCGGCACC	50.0	50.0	57.6	57.7	273 bp	1.0	75.5