Hotspot

rpl16-rps3

LSC · 94606-94781 · 176 bp

Back to Pseudocodon

Pi 0

MarkerSeek score 53.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 176
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAACCAACGAGTCACACACT	GGCTTCCCCTACAAACCATT	45.0	50.0	57.6	57.8	311-312 bp	1.0	75.2
rpl16-rps3_p2	GCAAAGCGATGAGTGGGTTA	GGCTTCCCCTACAAACCATT	50.0	50.0	58.6	57.8	392-393 bp	1.0	73.9
rpl16-rps3_p3	AAACCAACGAGTCACACACT	CCCTACAAACCATTCGAGCT	45.0	50.0	57.6	57.6	305-306 bp	1.0	73.9
rpl16-rps3_p4	GCAAAGCGATGAGTGGGTTA	CCCTACAAACCATTCGAGCT	50.0	50.0	58.6	57.6	386-387 bp	1.0	72.6
rpl16-rps3_p5	AAACCAACGAGTCACACACT	CCCCTACAAACCATTCGAGC	45.0	55.0	57.6	58.6	306-307 bp	1.0	72.1