Hotspot

ndhJ-petD

LSC · 68631-69244 · 614 bp

Back to Pseudocodon

Pi 0.00163

MarkerSeek score 59.70

Primer available yes

Label ndhJ-petD

Feature Metadata

label_name: ndhJ-petD
feature_type: igs
region: LSC
length_bp: 614
variable_sites: 1
pi: 0.00163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-petD_p1	TTTCATGCCGGAAAGTTGGA	CCCATTTCGTCGTCCAGTAG	45.0	55.0	58.0	58.1	817 bp	1.0	79.4
ndhJ-petD_p2	TTTCATGCCGGAAAGTTGGA	TTGGTTGGGTATTGGTGCAA	45.0	45.0	58.0	57.9	760 bp	1.0	79.3
ndhJ-petD_p3	TTTCATGCCGGAAAGTTGGA	TTTGGTTGGGTATTGGTGCA	45.0	45.0	58.0	57.9	761 bp	1.0	79.3
ndhJ-petD_p4	TTTCATGCCGGAAAGTTGGA	CAGTAGCGACAACCGTCTTT	45.0	50.0	58.0	58.2	803 bp	1.0	78.2
ndhJ-petD_p5	CGGATTTTCATGCCGGAAAG	CCCATTTCGTCGTCCAGTAG	50.0	55.0	57.8	58.1	822 bp	1.0	77.9