Hotspot

petA-psbJ

LSC · 63642-64675 · 1034 bp

Back to Pseudocodon

Pi 0

MarkerSeek score 59.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1034
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCAGCTGTTATTTTGGCAC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.3	57.6	1138-1145 bp	1.0	75.3
petA-psbJ_p2	GCAGCTGTTATTTTGGCACA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1137-1144 bp	1.0	74.1
petA-psbJ_p3	TTGGCAGCTGTTATTTTGGC	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1140-1147 bp	1.0	73.9
petA-psbJ_p4	TGGCAGCTGTTATTTTGGCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.7	57.6	1139-1146 bp	1.0	72.3
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.2	57.6	1171-1178 bp	1.0	71.5