Hotspot

rpoC2-rps2

LSC · 41034-41282 · 249 bp

Back to Pseudocodon

Pi 0.012

MarkerSeek score 59.40

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 249
variable_sites: 3
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	TCTTCGAGGGGGAAATGAGA	AGGTGCCATTTTCGGATTCC	50.0	50.0	57.8	58.5	467 bp	1.0	74.3
rpoC2-rps2_p2	TTCGAGGGGGAAATGAGAGA	AGGTGCCATTTTCGGATTCC	50.0	50.0	57.8	58.5	465 bp	1.0	74.3
rpoC2-rps2_p3	TCTTCGAGGGGGAAATGAGA	CATGGCCAAAATGAACTCCC	50.0	50.0	57.8	57.3	435 bp	1.0	73.1
rpoC2-rps2_p4	TTCGAGGGGGAAATGAGAGA	CATGGCCAAAATGAACTCCC	50.0	50.0	57.8	57.3	433 bp	1.0	73.1
rpoC2-rps2_p5	TCGAGGGGGAAATGAGAGAT	AGGTGCCATTTTCGGATTCC	50.0	50.0	57.5	58.5	464 bp	1.0	72.5