Hotspot

trnfM-CAU-rpl32

LSC · 16996-18349 · 1354 bp

Back to Pseudocodon

Pi 0.00171
MarkerSeek score 62.10
Primer available yes
Label trnfM-CAU-rpl32

Feature Metadata

label_name
trnfM-CAU-rpl32
feature_type
igs
region
LSC
length_bp
1354
variable_sites
2
pi
0.00171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-rpl32_p1 TCATCGGTTCAAATCCGGTC ATGCCAAAGCTTTTAACGCC 50.0 45.0 58.0 57.9 1296-1481 bp 1.0 83.3
trnfM-CAU-rpl32_p2 CATCGGTTCAAATCCGGTCT ATGCCAAAGCTTTTAACGCC 50.0 45.0 58.0 57.9 1295-1480 bp 1.0 83.3
trnfM-CAU-rpl32_p3 TCATCGGTTCAAATCCGGTC AGCCCTTCCCTTTCCAAATC 50.0 50.0 58.0 57.8 1269-1454 bp 1.0 82.1
trnfM-CAU-rpl32_p4 TCATCGGTTCAAATCCGGTC GAGCCCTTCCCTTTCCAAAT 50.0 50.0 58.0 57.8 1270-1455 bp 1.0 82.1
trnfM-CAU-rpl32_p5 TCATCGGTTCAAATCCGGTC GCCCTTCCCTTTCCAAATCT 50.0 50.0 58.0 57.8 1268-1453 bp 1.0 82.1