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trnQ-UUG-petN

LSC · 2834-5815 · 2982 bp

Back to Pseudocodon

Pi 0.000681

MarkerSeek score 57.50

Primer available yes

Label trnQ-UUG-petN

Feature Metadata

label_name: trnQ-UUG-petN
feature_type: igs
region: LSC
length_bp: 2982
variable_sites: 2
pi: 0.000681
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-petN_p1	GCTGGTATCACATCTCCGTC	CATTGGTCGAAGAGGCTCAG	55.0	55.0	57.9	58.4	280 bp	1.0	76.4
trnQ-UUG-petN_p2	GTCCGCTGGTATCACATCTC	CATTGGTCGAAGAGGCTCAG	55.0	55.0	57.9	58.4	284 bp	1.0	76.3
trnQ-UUG-petN_p3	GGAGATTGTCCGCTGGTATC	CATTGGTCGAAGAGGCTCAG	55.0	55.0	57.8	58.4	291 bp	1.0	76.2
trnQ-UUG-petN_p4	GCTGGTATCACATCTCCGTC	ATTGGTCGAAGAGGCTCAGA	55.0	50.0	57.9	58.4	279 bp	1.0	75.6
trnQ-UUG-petN_p5	GTCCGCTGGTATCACATCTC	ATTGGTCGAAGAGGCTCAGA	55.0	50.0	57.9	58.4	283 bp	1.0	75.6