Hotspot

trnH-GUG-psbA

LSC · 77-436 · 360 bp

Back to Pseudocodon

Pi 0

MarkerSeek score 50.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 360
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.66
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TCACAATCCACTGCCTTGTC	TAACCGGGCTAACCTTGGTA	50.0	50.0	58.1	58.1	497 bp	1.0	82.8
trnH-GUG-psbA_p2	TCACAATCCACTGCCTTGTC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.1	57.9	445 bp	1.0	82.6
trnH-GUG-psbA_p3	TTCACAATCCACTGCCTTGT	TAACCGGGCTAACCTTGGTA	45.0	50.0	57.6	58.1	498 bp	1.0	80.7
trnH-GUG-psbA_p4	TTCACAATCCACTGCCTTGT	TTCCCTCTAGACCTAGCTGC	45.0	55.0	57.6	57.9	446 bp	1.0	80.5
trnH-GUG-psbA_p5	TCACAATCCACTGCCTTGTC	ATTAACCGGGCTAACCTTGG	50.0	50.0	58.1	57.6	499 bp	1.0	79.7