Hotspot

secG-ycf45

LSC · 110413-110718 · 306 bp

Back to Pseudo-nitzschia

Pi 0.402

MarkerSeek score 75.40

Primer available yes

Label secG-ycf45

Feature Metadata

label_name: secG-ycf45
feature_type: igs
region: LSC
length_bp: 306
variable_sites: 197
pi: 0.402
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
secG-ycf45_p1	GTCTGGAAACCCCGAAGAAG	GGCACGAACTATTGCAGAGA	55.0	50.0	58.2	58.0	1751 bp	0.2	46.4
secG-ycf45_p2	GTCTGGAAACCCCGAAGAAG	ATGTTGCGACATCAGCAGAT	55.0	45.0	58.2	58.0	1509 bp	0.2	46.2
secG-ycf45_p3	GTCTGGAAACCCCGAAGAAG	AATGTTGCGACATCAGCAGA	55.0	45.0	58.2	58.2	1510 bp	0.2	45.0
secG-ycf45_p4	GTCTGGAAACCCCGAAGAAG	TGTTGCGACATCAGCAGATT	55.0	45.0	58.2	58.2	1508 bp	0.2	45.0
secG-ycf45_p5	ATTGTAACAACACGTGCTGC	GGCACGAACTATTGCAGAGA	45.0	50.0	57.6	58.0	1453 bp	0.2	44.7