Hotspot

trnF(gaa)-ycf90

LSC · 97902-98264 · 363 bp

Back to Pseudo-nitzschia

Pi 0.334

MarkerSeek score 77.70

Primer available yes

Label trnF(gaa)-ycf90

Feature Metadata

label_name: trnF(gaa)-ycf90
feature_type: igs
region: LSC
length_bp: 363
variable_sites: 201
pi: 0.334
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF(gaa)-ycf90_p1	GATCCTCGTGTCACCAGTTC	GACCGCGTCCAAAGAATAGT	55.0	50.0	58.0	58.0	600-1030 bp	0.8	82.3
trnF(gaa)-ycf90_p2	TCAGTTGGTAGAGCAGTGGA	GACCGCGTCCAAAGAATAGT	50.0	50.0	58.0	58.0	625-1055 bp	0.8	82.2
trnF(gaa)-ycf90_p3	ATTCCTGGGAACGATGATGC	GACCGCGTCCAAAGAATAGT	50.0	50.0	58.0	58.0	871 bp	0.2	47.7
trnF(gaa)-ycf90_p4	GCACAGGAAGCAAAACGATC	GACCGCGTCCAAAGAATAGT	50.0	50.0	58.0	58.0	1228 bp	0.2	47.7
trnF(gaa)-ycf90_p5	GATCCTCGTGTCACCAGTTC	ATGGCAGGATGAGAAGCAAG	55.0	50.0	58.0	57.9	557 bp	0.2	47.5