Hotspot

trnT(tgt)-trnR(tct)

LSC · 74526-74753 · 228 bp

Back to Pseudo-nitzschia

Pi 0.43

MarkerSeek score 80.50

Primer available yes

Label trnT(tgt)-trnR(tct)

Feature Metadata

label_name: trnT(tgt)-trnR(tct)
feature_type: igs
region: LSC
length_bp: 228
variable_sites: 160
pi: 0.43
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT(tgt)-trnR(tct)_p1	AACCCCCAACCTACGGATTA	TTCGATTCCTACTGGGCCTA	50.0	50.0	58.0	57.5	288-382 bp	1.0	88.0
trnT(tgt)-trnR(tct)_p2	ATTTGAACCCCCAACCTACG	TTCGATTCCTACTGGGCCTA	50.0	50.0	57.8	57.5	293-387 bp	1.0	86.6
trnT(tgt)-trnR(tct)_p3	ACAAAACCGTTGCTCTACCA	TTCGATTCCTACTGGGCCTA	45.0	50.0	57.7	57.5	269-363 bp	1.0	85.6
trnT(tgt)-trnR(tct)_p4	ACCGTTGCTCTACCATTGAG	TTCGATTCCTACTGGGCCTA	50.0	50.0	57.6	57.5	264-358 bp	1.0	85.2
trnT(tgt)-trnR(tct)_p5	AACCCCCAACCTACGGATTA	GTTCGATTCCTACTGGGCCT	50.0	55.0	58.0	59.2	289-383 bp	1.0	82.4