Hotspot

psbD-psbK

LSC · 73588-73918 · 331 bp

Back to Pseudo-nitzschia

Pi 0.193

MarkerSeek score 82.00

Primer available yes

Label psbD-psbK

Feature Metadata

label_name: psbD-psbK
feature_type: igs
region: LSC
length_bp: 331
variable_sites: 106
pi: 0.193
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-psbK_p1	AAACCTGACCAACCAACGAA	ACGTATGCTTCTGGTAAACGA	45.0	42.9	57.9	57.4	459-474 bp	1.0	78.3
psbD-psbK_p2	GCCACGTTCTTGGTTTTGTC	ACGTATGCTTCTGGTAAACGA	50.0	42.9	58.2	57.4	394-409 bp	1.0	78.0
psbD-psbK_p3	AACCTGACCAACCAACGAAT	ACGTATGCTTCTGGTAAACGA	45.0	42.9	57.6	57.4	458-473 bp	1.0	76.5
psbD-psbK_p4	CCACGTTCTTGGTTTTGTCC	ACGTATGCTTCTGGTAAACGA	50.0	42.9	57.5	57.4	393-408 bp	1.0	75.5
psbD-psbK_p5	AGCCACGTTCTTGGTTTTGT	ACGTATGCTTCTGGTAAACGA	45.0	42.9	58.5	57.4	395-410 bp	1.0	75.1