Hotspot

trnI(gat)-rns

IRa · 63009-63090 · 82 bp

Back to Pseudo-nitzschia

Pi 0.112

MarkerSeek score 80.60

Primer available yes

Label trnI(gat)-rns

Feature Metadata

label_name: trnI(gat)-rns
feature_type: igs
region: IRa
length_bp: 82
variable_sites: 18
pi: 0.112
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI(gat)-rns_p1	TACCAGCTGAGCTATACCCC	GTAACCGTACTGGAAGGTGC	55.0	55.0	58.0	58.3	217-241 bp	1.0	84.2
trnI(gat)-rns_p2	TACCAGCTGAGCTATACCCC	AGGTAACCGTACTGGAAGGT	55.0	50.0	58.0	57.7	219-243 bp	1.0	83.9
trnI(gat)-rns_p3	TACCAGCTGAGCTATACCCC	AGTGACTAGGGTGAAGTCGT	55.0	50.0	58.0	57.7	243-267 bp	1.0	83.5
trnI(gat)-rns_p4	TACCAGCTGAGCTATACCCC	GGTAACCGTACTGGAAGGTG	55.0	55.0	58.0	57.6	218-242 bp	1.0	83.5
trnI(gat)-rns_p5	TACCAGCTGAGCTATACCCC	GGAGGAGGATGCCTAAGGTA	55.0	55.0	58.0	57.6	268-292 bp	1.0	82.1