Hotspot

rrn5-rnl

IRa · 59896-59946 · 51 bp

Back to Pseudo-nitzschia

Pi 0.0745

MarkerSeek score 80.30

Primer available yes

Label rrn5-rnl

Feature Metadata

label_name: rrn5-rnl
feature_type: igs
region: IRa
length_bp: 51
variable_sites: 7
pi: 0.0745
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rnl_p1	GTGGTTCCGCTGAATACACT	GATGTGCTAACAGACCGAGG	50.0	55.0	57.9	58.1	105-106 bp	1.0	84.7
rrn5-rnl_p2	CAGTGTGGTTCCGCTGAATA	GATGTGCTAACAGACCGAGG	50.0	55.0	57.9	58.1	109-110 bp	1.0	84.5
rrn5-rnl_p3	AGTGTGGTTCCGCTGAATAC	GATGTGCTAACAGACCGAGG	50.0	55.0	57.9	58.1	108-109 bp	1.0	84.5
rrn5-rnl_p4	TGTGGTTCCGCTGAATACAC	GATGTGCTAACAGACCGAGG	50.0	55.0	58.2	58.1	106-107 bp	1.0	83.8
rrn5-rnl_p5	TCGAGATGGATCAGTGTGGT	GATGTGCTAACAGACCGAGG	50.0	55.0	58.1	58.1	120-121 bp	1.0	83.5