Hotspot

rps16-rrn5

IRa · 59161-59767 · 607 bp

Back to Pseudo-nitzschia

Pi 0.459

MarkerSeek score 81.90

Primer available yes

Label rps16-rrn5

Feature Metadata

label_name: rps16-rrn5
feature_type: igs
region: IRa
length_bp: 607
variable_sites: 448
pi: 0.459
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-rrn5_p1	AAACGAATTGGCCGAAAACG	GCTAACAGACCGAGGACTTG	45.0	55.0	58.2	58.0	1017-1170 bp	0.4	63.9
rps16-rrn5_p2	AGAAGCACGTCGAAGAAAGG	GCTAACAGACCGAGGACTTG	50.0	55.0	58.2	58.0	1464 bp	0.2	46.3
rps16-rrn5_p3	CTCAACCAACGGAGACTGTT	GCTAACAGACCGAGGACTTG	50.0	55.0	57.8	58.0	854 bp	0.2	46.1
rps16-rrn5_p4	AGAAGCACGTCGAAGAAAGG	ATCTCCCCCAAGAGTTCACA	50.0	50.0	58.2	58.0	1880 bp	0.2	45.9
rps16-rrn5_p5	AGAAGCACGTCGAAGAAAGG	TCTCCCCCAAGAGTTCACAT	50.0	50.0	58.2	58.0	1879 bp	0.2	45.9