Hotspot

trnC(gca)-psbY

SSC · 53778-54526 · 749 bp

Back to Pseudo-nitzschia

Pi 0.381

MarkerSeek score 81.00

Primer available yes

Label trnC(gca)-psbY

Feature Metadata

label_name: trnC(gca)-psbY
feature_type: igs
region: SSC
length_bp: 749
variable_sites: 450
pi: 0.381
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC(gca)-psbY_p1	CCTTACCACTTGGCCATACC	ATAATGCCCATGATGCTGCA	55.0	45.0	57.9	58.0	555-2838 bp	1.0	91.5
trnC(gca)-psbY_p2	CCTTACCACTTGGCCATACC	GGTGATGAAGCAATTTGGCC	55.0	50.0	57.9	58.0	943-1613 bp	0.6	75.5
trnC(gca)-psbY_p3	CCTTACCACTTGGCCATACC	ACACGTGGTGATGAAGCAAT	55.0	45.0	57.9	58.1	1223-1619 bp	0.4	66.7
trnC(gca)-psbY_p4	CCTTACCACTTGGCCATACC	ACAGGCTGTTTCACATCGTT	55.0	45.0	57.9	58.0	1263 bp	0.2	47.3
trnC(gca)-psbY_p5	CCTTACCACTTGGCCATACC	TCTGTTGGACAGGCTGTTTC	55.0	50.0	57.9	58.0	1271 bp	0.2	47.3