Hotspot

rrn5-rps10

IRb · 19215-19918 · 704 bp

Back to Pseudo-nitzschia

Pi 0.433

MarkerSeek score 81.10

Primer available yes

Label rrn5-rps10

Feature Metadata

label_name: rrn5-rps10
feature_type: igs
region: IRb
length_bp: 704
variable_sites: 494
pi: 0.433
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rps10_p1	GCTAACAGACCGAGGACTTG	TTACCCTGTTGCGATCGAAG	55.0	50.0	58.0	58.0	1181-1328 bp	0.4	67.9
rrn5-rps10_p2	ATCTCCCCCAAGAGTTCACA	TTACCCTGTTGCGATCGAAG	50.0	50.0	58.0	58.0	1597-1744 bp	0.4	67.6
rrn5-rps10_p3	TCTCCCCCAAGAGTTCACAT	TTACCCTGTTGCGATCGAAG	50.0	50.0	58.0	58.0	1596-1743 bp	0.4	67.6
rrn5-rps10_p4	TTCGGTCCATATCCGGTGTA	TTACCCTGTTGCGATCGAAG	50.0	50.0	57.9	58.0	1452-1599 bp	0.4	67.4
rrn5-rps10_p5	GATGTGCTAACAGACCGAGG	TTACCCTGTTGCGATCGAAG	55.0	50.0	58.1	58.0	1186-1333 bp	0.4	67.4