Hotspot

rns-trnI(gat)

IRb · 15892-15973 · 82 bp

Back to Pseudo-nitzschia

Pi 0.107

MarkerSeek score 80.60

Primer available yes

Label rns-trnI(gat)

Feature Metadata

label_name: rns-trnI(gat)
feature_type: igs
region: IRb
length_bp: 82
variable_sites: 17
pi: 0.107
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-trnI(gat)_p1	GTAACCGTACTGGAAGGTGC	TACCAGCTGAGCTATACCCC	55.0	55.0	58.3	58.0	217-241 bp	1.0	83.9
rns-trnI(gat)_p2	AGGTAACCGTACTGGAAGGT	TACCAGCTGAGCTATACCCC	50.0	55.0	57.7	58.0	219-243 bp	1.0	83.6
rns-trnI(gat)_p3	AGTGACTAGGGTGAAGTCGT	TACCAGCTGAGCTATACCCC	50.0	55.0	57.7	58.0	243-267 bp	1.0	83.2
rns-trnI(gat)_p4	GGTAACCGTACTGGAAGGTG	TACCAGCTGAGCTATACCCC	55.0	55.0	57.6	58.0	218-242 bp	1.0	83.1
rns-trnI(gat)_p5	GGAGGAGGATGCCTAAGGTA	TACCAGCTGAGCTATACCCC	55.0	55.0	57.6	58.0	268-292 bp	1.0	81.8