Hotspot

ycf2

LSC · 35561-42403 · 6843 bp

Back to Pseudalcantarea

Pi 0.00205

MarkerSeek score 53.70

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 6843
variable_sites: 14
pi: 0.00205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CCTGTGACTCGATTGGTGAA	AATTGGCTGAATCCCGTGAA	50.0	45.0	57.5	57.8	283 bp	1.0	75.5
ycf2_p2	CCTGTGACTCGATTGGTGAA	ATTGGCTGAATCCCGTGAAA	50.0	45.0	57.5	57.8	282 bp	1.0	75.5
ycf2_p3	CCTGTGACTCGATTGGTGAA	AGACAATTGGCTGAATCCCG	50.0	50.0	57.5	58.2	287 bp	1.0	75.2
ycf2_p4	ACCTGTGACTCGATTGGTGA	AATTGGCTGAATCCCGTGAA	50.0	45.0	58.7	57.8	284 bp	1.0	73.8
ycf2_p5	ACCTGTGACTCGATTGGTGA	ATTGGCTGAATCCCGTGAAA	50.0	45.0	58.7	57.8	283 bp	1.0	73.8