Hotspot

rps19

LSC · 275-556 · 282 bp

Back to Psathyrostachys

Pi NA

MarkerSeek score 29.60

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 282
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 29.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TTCTGACCTCCGTACTTCGA	TCTTAATCTTTCTATTCAGAATTCAG	50.0	26.9	57.8	53.5	385 bp	1.0	44.0
rps19_p2	TCTGACCTCCGTACTTCGAT	TCTTAATCTTTCTATTCAGAATTCAG	50.0	26.9	57.6	53.5	384 bp	1.0	44.0
rps19_p3	CCTCCGTACTTCGATCGAGA	TCTTAATCTTTCTATTCAGAATTCAG	55.0	26.9	58.4	53.5	379 bp	1.0	44.0
rps19_p4	TTCTGACCTCCGTACTTCGA	CTTCTTAATCTTTCTATTCAGAATTCA	50.0	25.9	57.8	54.2	387 bp	1.0	44.0
rps19_p5	TTCTGACCTCCGTACTTCGA	TTCTTAATCTTTCTATTCAGAATTCAG	50.0	25.9	57.8	54.2	386 bp	1.0	44.0