Hotspot

rps18

LSC · 66411-66923 · 513 bp

Back to Psathyrostachys

Pi 0.0117

MarkerSeek score 63.70

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 513
variable_sites: 6
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AGACTACTTCTTCTGGATCCT	GGTAGGAATCAACGAGCTGG	42.9	55.0	54.8	58.1	674-675 bp	1.0	46.9
rps18_p2	AGACTACTTCTTCTGGATCCT	AGGGAACTCCGGGAAGATAC	42.9	55.0	54.8	58.2	712-713 bp	1.0	45.7
rps18_p3	AAGACTACTTCTTCTGGATCCT	GGTAGGAATCAACGAGCTGG	40.9	55.0	55.6	58.1	675-676 bp	1.0	45.4
rps18_p4	AAGACTACTTCTTCTGGATCCT	AGGGAACTCCGGGAAGATAC	40.9	55.0	55.6	58.2	713-714 bp	1.0	44.1
rps18_p5	AGACTACTTCTTCTGGATCCT	TAGGTCTGGCCCTTTCGAAT	42.9	50.0	54.8	58.4	632-633 bp	1.0	44.1