Hotspot

rpl16

LSC · 78406-79866 · 1461 bp

Back to Psathyrostachys

Pi 0.00219

MarkerSeek score 64.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1461
variable_sites: 3
pi: 0.00219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCGGGC	TCCTGAAATCCCTGCAAAAGA	50.0	42.9	58.7	57.5	1513-1601 bp	1.0	66.6
rpl16_p2	CCAGAAAGAAAAACCGGGCA	TCCTGAAATCCCTGCAAAAGA	50.0	42.9	59.0	57.5	1512-1600 bp	1.0	64.3
rpl16_p3	CAGAAAGAAAAACCGGGCAT	TCCTGAAATCCCTGCAAAAGA	45.0	42.9	56.9	57.5	1511-1599 bp	1.0	63.1
rpl16_p4	TCCAGAAAGAAAAACCGGGC	CCTGAAATCCCTGCAAAAGA	50.0	45.0	58.7	55.9	1512-1600 bp	1.0	62.0
rpl16_p5	TCCAGAAAGAAAAACCGGGC	TCCTGAAATCCCTGCAAAAG	50.0	45.0	58.7	55.9	1513-1601 bp	1.0	62.0