Hotspot

rbcL-psaI

LSC · 56470-58145 · 1676 bp

Back to Psathyrostachys

Pi 0.0133

MarkerSeek score 55.90

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1676
variable_sites: 21
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CCGAAATCTTTGGGGACGAT	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.0	57.9	1929-2010 bp	1.0	84.0
rbcL-psaI_p2	GATCATATCCACGCCGGTAC	GTCATTGCAATTGCCGGAAA	55.0	45.0	58.0	57.9	2131-2212 bp	1.0	83.8
rbcL-psaI_p3	ATATCCACGCCGGTACAGTA	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.0	57.9	2127-2208 bp	1.0	83.7
rbcL-psaI_p4	GATCATATCCACGCCGGTAC	CGCATCCATAGCCGTTTTTC	55.0	50.0	58.0	57.9	2263-2344 bp	1.0	83.3
rbcL-psaI_p5	GATCATATCCACGCCGGTAC	TGAAATCGCCTTCAGCAACT	55.0	45.0	58.0	58.1	2421-2502 bp	1.0	83.1