Hotspot

trnF-GAA-ndhJ

LSC · 48309-48898 · 590 bp

Back to Psathyrostachys

Pi 0.0328

MarkerSeek score 64.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 19
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GGATTTGGAGAAGTGCCGAT	50.0	50.0	58.1	58.0	786-797 bp	1.0	82.4
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GGCTTTGATCACCGAGGAAT	50.0	50.0	58.1	58.0	1033-1044 bp	1.0	81.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TTTACGCTCCCAATGTGCTT	50.0	45.0	58.1	58.1	941-952 bp	1.0	81.4
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GGGATTGGGATTCCATTGCT	50.0	50.0	58.1	57.9	990-1001 bp	1.0	81.3
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	GGAGGGGCTCTTTTGTGTAG	50.0	55.0	58.1	57.9	1192-1203 bp	1.0	80.7