Hotspot

petN-trnC-GCA

LSC · 18311-19031 · 721 bp

Back to Psathyrostachys

Pi 0.0154

MarkerSeek score 62.20

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 11
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGTCCACTCCTTCCCCATAC	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	829-1042 bp	1.0	72.1
petN-trnC-GCA_p2	GTCCACTCCTTCCCCATACT	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	828-1041 bp	1.0	72.1
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	770-983 bp	1.0	71.9
petN-trnC-GCA_p4	AGTCCACTCCTTCCCCATAC	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	831-1044 bp	1.0	71.6
petN-trnC-GCA_p5	GTCCACTCCTTCCCCATACT	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	830-1043 bp	1.0	71.6