Hotspot

trnG-UCC-trnT-GGU

LSC · 14190-15385 · 1196 bp

Back to Psathyrostachys

Pi 0.0151

MarkerSeek score 58.50

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1196
variable_sites: 18
pi: 0.0151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	TGACTTACGTCTTAGCACGAG	38.1	47.6	55.5	57.5	1274-1275 bp	1.0	41.5
trnG-UCC-trnT-GGU_p2	AGAACGAATCACACTTTTACCAC	TGACTTACGTCTTAGCACGAG	39.1	47.6	57.3	57.5	1277-1278 bp	1.0	41.5
trnG-UCC-trnT-GGU_p3	GAACGAATCACACTTTTACCACT	TGACTTACGTCTTAGCACGAG	39.1	47.6	57.3	57.5	1276-1277 bp	1.0	41.5
trnG-UCC-trnT-GGU_p4	ACGAATCACACTTTTACCACT	TGACTTACGTCTTAGCACGA	38.1	45.0	55.5	56.4	1274-1275 bp	1.0	41.5
trnG-UCC-trnT-GGU_p5	AACGAATCACACTTTTACCACT	TGACTTACGTCTTAGCACGAG	36.4	47.6	56.2	57.5	1275-1276 bp	1.0	41.5