Hotspot

rps19-psbA

LSC · 557-686 · 130 bp

Back to Psathyrostachys

Pi 0.0482

MarkerSeek score 74.10

Primer available yes

Label rps19-psbA

Feature Metadata

label_name: rps19-psbA
feature_type: igs
region: LSC
length_bp: 130
variable_sites: 4
pi: 0.0482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.64
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-psbA_p1	CAAATTGGGGGAATTCGTGC	TGGAAGTAATGCACGAACGT	50.0	45.0	58.0	57.8	289 bp	0.5	58.7
rps19-psbA_p2	GCAAATTGGGGGAATTCGTG	TGGAAGTAATGCACGAACGT	50.0	45.0	58.0	57.8	290 bp	0.5	58.7
rps19-psbA_p3	CAAATTGGGGGAATTCGTGC	CAACCGTGCTAACCTTGGTA	50.0	50.0	58.0	57.8	309 bp	0.5	58.5
rps19-psbA_p4	GCAAATTGGGGGAATTCGTG	CAACCGTGCTAACCTTGGTA	50.0	50.0	58.0	57.8	310 bp	0.5	58.5
rps19-psbA_p5	CAAATTGGGGGAATTCGTGC	ATCAACCGTGCTAACCTTGG	50.0	50.0	58.0	58.2	311 bp	0.5	58.5