Hotspot

rpl32-trnL-UAG

SSC · 115736-117159 · 1424 bp

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Pi 0.0217
MarkerSeek score 64.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1424
variable_sites
260
pi
0.0217
unique_haplotype_count
88
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGATATCGGGCAGCATTGAA ATCCACACAAAAGAGCTGCA 50.0 45.0 58.1 58.0 275-2186 bp 1.0 91.1
rpl32-trnL-UAG_p2 GGATATCGGGCAGCATTGAA TCCACACAAAAGAGCTGCAT 50.0 45.0 58.1 58.0 274-2185 bp 1.0 91.1
rpl32-trnL-UAG_p3 GGGCAGCATTGAAAGCTTTT ATCCACACAAAAGAGCTGCA 45.0 45.0 57.8 58.0 268-2179 bp 1.0 90.5
rpl32-trnL-UAG_p4 GGGCAGCATTGAAAGCTTTT TCCACACAAAAGAGCTGCAT 45.0 45.0 57.8 58.0 267-2178 bp 1.0 90.5
rpl32-trnL-UAG_p5 GGATATCGGGCAGCATTGAA AGAGCAGCGTGTCTACCTAT 50.0 50.0 58.1 57.9 274-1592 bp 0.808333 83.0