Hotspot

psaJ-rpl33

LSC · 69009-69469 · 461 bp

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Pi 0.0127
MarkerSeek score 60.60
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
461
variable_sites
56
pi
0.0127
unique_haplotype_count
68
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TCCGGATGCGTTGATATTCC GGACATCTTTACCCTTGGCC 50.0 55.0 57.8 58.2 514-573 bp 1.0 88.8
psaJ-rpl33_p2 CGGGTCTTTGGCAGGTTTAT GGACATCTTTACCCTTGGCC 50.0 55.0 58.2 58.2 556-615 bp 1.0 88.8
psaJ-rpl33_p3 TTATGGTTCGGGTCTTTGGC GGACATCTTTACCCTTGGCC 50.0 55.0 58.2 58.2 564-623 bp 0.991667 88.4
psaJ-rpl33_p4 GTGCTTTATGGTTCGGGTCT GGACATCTTTACCCTTGGCC 50.0 55.0 57.9 58.2 569-628 bp 0.966667 87.7
psaJ-rpl33_p5 AGTGCTTTATGGTTCGGGTC GGACATCTTTACCCTTGGCC 50.0 55.0 57.9 58.2 570-629 bp 0.966667 87.6