Hotspot

ndhC-trnV-UAC

LSC · 52454-53076 · 623 bp

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Pi 0.0284
MarkerSeek score 65.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
623
variable_sites
151
pi
0.0284
unique_haplotype_count
76
primer_available
yes
alignment_reliability
0.96
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ACTATCAGAAATGCCCAGAA AGAAGGTCTACGGTTCGAGT 40.0 50.0 54.0 57.8 456-757 bp 1.0 58.5
ndhC-trnV-UAC_p2 ACTATCAGAAATGCCCAGAAA AGAAGGTCTACGGTTCGAGT 38.1 50.0 54.9 57.8 456-757 bp 1.0 57.5
ndhC-trnV-UAC_p3 TGATACTATCAGAAATGCCCAGA AGAAGGTCTACGGTTCGAGT 39.1 50.0 56.8 57.8 460-761 bp 1.0 57.2
ndhC-trnV-UAC_p4 ACTTGATACTATCAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 37.5 50.0 57.7 57.8 463-764 bp 1.0 56.4
ndhC-trnV-UAC_p5 ACTATCAGAAATGCCCAGAAAA AGAAGGTCTACGGTTCGAGT 36.4 50.0 55.7 57.8 456-757 bp 1.0 55.9