Hotspot

trnL-UAA-trnF-GAA

LSC · 49543-49927 · 385 bp

Back to Prunus

Pi 0.0176
MarkerSeek score 56.70
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
385
variable_sites
56
pi
0.0176
unique_haplotype_count
45
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TCTACCAGCTGAGCTATCCC 50.0 55.0 57.5 58.0 265-1643 bp 1.0 88.2
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 301-1679 bp 1.0 87.8
trnL-UAA-trnF-GAA_p3 AATCGTGAGGGTTCAAGTCC GTGCCAGGAACCAGATTTGA 50.0 50.0 57.5 58.1 316-1694 bp 1.0 87.5
trnL-UAA-trnF-GAA_p4 AATCGTGAGGGTTCAAGTCC CATGTGCCAGGAACCAGATT 50.0 50.0 57.5 58.2 319-1697 bp 1.0 87.0
trnL-UAA-trnF-GAA_p5 AATCGTGAGGGTTCAAGTCC TCAGTCCTCTGCTCTACCAG 50.0 55.0 57.5 57.9 277-1655 bp 0.958333 85.4