Hotspot

trnC-GCA-petN

LSC · 28327-29216 · 890 bp

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Pi 0.00949
MarkerSeek score 60.80
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
890
variable_sites
119
pi
0.00949
unique_haplotype_count
63
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCGAGATTTACT 50.0 50.0 58.1 58.0 763-1024 bp 1.0 91.1
trnC-GCA-petN_p2 GGGGGACTGCAAATCCTTTT CAGCCCAAGCGAGATTTACT 50.0 50.0 58.4 58.0 781-1042 bp 1.0 88.9
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 823-1084 bp 1.0 88.5
trnC-GCA-petN_p4 GGGGGACTGCAAATCCTTTT AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.4 57.6 841-1102 bp 1.0 86.3
trnC-GCA-petN_p5 CCAGTTCAAATCCGGGTGTC CAGCCCAAGCGAGATTTACT 55.0 50.0 58.8 58.0 758-1019 bp 1.0 85.1