Hotspot

psbI-trnS-GCU

LSC · 8045-8181 · 137 bp

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Pi 0.029
MarkerSeek score 63.00
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
137
variable_sites
27
pi
0.029
unique_haplotype_count
29
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CGTAATCCTGGACGTGAAGA TGGACTAAAGCGTCGGATTG 50.0 50.0 57.1 58.0 228-246 bp 1.0 83.6
psbI-trnS-GCU_p2 CGTAATCCTGGACGTGAAGA GTGGACTAAAGCGTCGGATT 50.0 50.0 57.1 58.0 229-247 bp 1.0 83.5
psbI-trnS-GCU_p3 CGTAATCCTGGACGTGAAGA CAATTGGGAGGAGAGATGGC 50.0 55.0 57.1 58.0 252-270 bp 1.0 83.1
psbI-trnS-GCU_p4 CGTAATCCTGGACGTGAAGA AATTGGGAGGAGAGATGGCT 50.0 50.0 57.1 58.1 251-269 bp 1.0 82.6
psbI-trnS-GCU_p5 CGTAATCCTGGACGTGAAGA TTTTTCGTACCGAGGGTTCG 50.0 50.0 57.1 58.2 191-209 bp 1.0 82.4