Hotspot

rbcL

LSC · 54705-56138 · 1434 bp

Back to Prunella

Pi 0.00209

MarkerSeek score 60.00

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1434
variable_sites: 3
pi: 0.00209
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	AGACCTTGTTGTTGTCCGAA	TGGGCCGAGTTTAATTGCAA	45.0	45.0	57.3	58.4	1545 bp	1.0	71.5
rbcL_p2	AGACCTTGTTGTTGTCCGAA	TTGGGCCGAGTTTAATTGCA	45.0	45.0	57.3	58.4	1546 bp	1.0	71.5
rbcL_p3	AGACCTTGTTGTTGTCCGAA	AAAATACGTGCAATGCGCTG	45.0	45.0	57.3	58.4	1603 bp	1.0	71.5
rbcL_p4	AGACCTTGTTGTTGTCCGAA	CAAAATACGTGCAATGCGCT	45.0	45.0	57.3	58.4	1604 bp	1.0	71.5
rbcL_p5	AACGCCCAATTCATTCGTGT	TGGGCCGAGTTTAATTGCAA	45.0	45.0	58.8	58.4	1570 bp	1.0	71.1