Hotspot

ndhK

LSC · 48884-49561 · 678 bp

Back to Prunella

Pi 0.00147

MarkerSeek score 59.60

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 678
variable_sites: 1
pi: 0.00147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	GATGAAAATTGACCCGCTGC	GTTTATGCATGGCGAAAGGG	50.0	50.0	58.1	57.8	817 bp	1.0	77.7
ndhK_p2	AATTGACCCGCTGCTTGTTA	GTTTATGCATGGCGAAAGGG	45.0	50.0	58.1	57.8	811 bp	1.0	77.7
ndhK_p3	TTGACCCGCTGCTTGTTATT	GTTTATGCATGGCGAAAGGG	45.0	50.0	58.1	57.8	809 bp	1.0	77.7
ndhK_p4	ATTGACCCGCTGCTTGTTAT	GTTTATGCATGGCGAAAGGG	45.0	50.0	57.9	57.8	810 bp	1.0	77.4
ndhK_p5	GACCCGCTGCTTGTTATTCT	GTTTATGCATGGCGAAAGGG	50.0	50.0	58.3	57.8	807 bp	1.0	76.3