Hotspot

ccsA

SSC · 111797-112774 · 978 bp

Back to Prunella

Pi 0.00204

MarkerSeek score 59.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 2
pi: 0.00204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCATGGCATGTCAACTCCTA	AAAGCCCCGTGACTTTGATT	50.0	45.0	58.0	58.0	1178 bp	1.0	79.8
ccsA_p2	GCATGGCATGTCAACTCCTA	AAGCCCCGTGACTTTGATTC	50.0	50.0	58.0	58.5	1177 bp	1.0	76.1
ccsA_p3	GCATGGCATGTCAACTCCTA	GAAAGCCCCGTGACTTTGAT	50.0	50.0	58.0	58.5	1179 bp	1.0	76.1
ccsA_p4	GCATGGCATGTCAACTCCTA	GGGGCTCTCGCTAGTTCATA	50.0	55.0	58.0	58.7	1155 bp	1.0	74.4
ccsA_p5	GCATGGCATGTCAACTCCTA	GAAAGCCCCGTGACTTTGA	50.0	52.6	58.0	58.0	1179 bp	1.0	71.7