Hotspot

rpl32-trnL-UAG

SSC · 111125-111618 · 494 bp

Back to Prunella

Pi 0.00607

MarkerSeek score 60.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 494
variable_sites: 3
pi: 0.00607
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGGAAAGGATATTGGGCGG	TAGGAGTTGACATGCCATGC	50.0	50.0	57.6	58.0	700 bp	1.0	77.1
rpl32-trnL-UAG_p2	AAGGAAAGGATATTGGGCGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.6	57.9	642 bp	1.0	76.5
rpl32-trnL-UAG_p3	GGCGGCGTTAAAAGCTTTTT	TAGGAGTTGACATGCCATGC	45.0	50.0	58.5	58.0	685 bp	1.0	76.0
rpl32-trnL-UAG_p4	AAGGAAAGGATATTGGGCGG	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.6	57.8	635 bp	1.0	76.0
rpl32-trnL-UAG_p5	AAGGAAAGGATATTGGGCGG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.6	57.8	640 bp	1.0	75.9