Hotspot

rpl20-rps12

LSC · 67814-68600 · 787 bp

Back to Prunella

Pi 0.00127
MarkerSeek score 59.50
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
787
variable_sites
1
pi
0.00127
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCCGAGCTATATATCCGCGT ACTAGGGTGTATGTGCGACT 50.0 50.0 58.0 58.2 845 bp 1.0 78.6
rpl20-rps12_p2 TCCGAGCTATATATCCGCGT ACAGCCCATTCGAAATGTCA 50.0 45.0 58.0 57.8 912 bp 1.0 78.2
rpl20-rps12_p3 TCCGAGCTATATATCCGCGT ACAAGACAGCCCATTCGAAA 50.0 45.0 58.0 57.7 917 bp 1.0 77.7
rpl20-rps12_p4 ACCCTCGAAAACTTGATGCA ACTAGGGTGTATGTGCGACT 45.0 50.0 57.7 58.2 890 bp 1.0 76.7
rpl20-rps12_p5 ACCCTCGAAAACTTGATGCA ACAGCCCATTCGAAATGTCA 45.0 45.0 57.7 57.8 957 bp 1.0 76.3