Hotspot

petL-petG

LSC · 64997-65181 · 185 bp

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Pi 0
MarkerSeek score 26.80
Primer available yes
Label petL-petG

Feature Metadata

label_name
petL-petG
feature_type
igs
region
LSC
length_bp
185
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
26.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petL-petG_p1 TGTCGTTTAGCCCCTTCATG CGCCACGCCTGTATTGTAAA 50.0 50.0 57.9 58.9 395 bp 1.0 71.8
petL-petG_p2 GATGTCGTTTAGCCCCTTCA CGCCACGCCTGTATTGTAAA 50.0 50.0 57.6 58.9 397 bp 1.0 69.5
petL-petG_p3 CAGATGTCGTTTAGCCCCTT CGCCACGCCTGTATTGTAAA 50.0 50.0 57.6 58.9 399 bp 1.0 69.4
petL-petG_p4 TGTCGTTTAGCCCCTTCATG CGCCACGCCTGTATTGTAA 50.0 52.6 57.9 58.2 395 bp 1.0 69.2
petL-petG_p5 CGTTTAGCCCCTTCATGCTT CGCCACGCCTGTATTGTAAA 50.0 50.0 58.5 58.9 392 bp 1.0 68.4