Hotspot

rbcL-accD

LSC · 56139-56803 · 665 bp

Back to Prunella

Pi 0.00451

MarkerSeek score 52.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 3
pi: 0.00451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GGAGTCCTGAATTAGCTGCC	GATACCCGCGTTCTAACTCC	55.0	55.0	58.0	57.9	806 bp	1.0	78.9
rbcL-accD_p2	CCGCTTGTGAGGTATGGAAA	GATACCCGCGTTCTAACTCC	50.0	55.0	57.9	57.9	788 bp	1.0	78.4
rbcL-accD_p3	GGAGTCCTGAATTAGCTGCC	ATACCCGCGTTCTAACTCCT	55.0	50.0	58.0	58.2	805 bp	1.0	78.2
rbcL-accD_p4	GGAGTCCTGAATTAGCTGCC	CGATACCCGCGTTCTAACTC	55.0	55.0	58.0	58.2	807 bp	1.0	78.0
rbcL-accD_p5	CCGCTTGTGAGGTATGGAAA	ATACCCGCGTTCTAACTCCT	50.0	50.0	57.9	58.2	787 bp	1.0	77.6